Monday, June 6, 2011

DYO #2: Fruit Fly Population Investigation

Problem (Question): How does putting four female and two male fruit flies affect how many adults are going to be after three weeks?


Hypothesis: If I put four females and two males in a vial then i expect to see 646 adults at the end of three weeks because each females lays 20 eggs each day. The eggs take 16 days to become adult flies. After three weeks a female would have 160 eggs, but we have 4 females and six fruit flies. We multiply the eggs a female would have after three weeks (160) by the number of female fruit flies in the vial (4), then add the fruit flies we started with (6). This would give us 646 adult fruit flies at the end of three weeks.

Background Research: The Fruit Fly has four stages of life; egg, larva, pupa, and adult. Each stage looks different. The egg looks like a grain of white rice, the larva is a tiny white worm, the pupa looks like a small cocoon & the adult looks like a miniature fly. A female can lay 20 eggs per day, and it takes 16 days for the egg to become an adult. You can tell the difference between a male and a female fruit fly by looking at its size,  the female is slightly bigger than a male fruit fly. Also by looking at its abdomen, male fruit flies have a darker tip and females have a lighter tip.

Procedure:
  • Step 1: First we chose to use the combination of 2 females and 1 male fruit fly.
  • Step 2: Then we put the flies to sleep using something called FlyNap.
  • Step 3: After we put the flies to sleep, we put food in the vial to make a habitat for the flies.
  • Step 4: Then we identified the female and the male fruit flies by looking by looking at their abdomens. 
  • Step 5: After we chose 2 females and 1 male fly, we put them in the vial and sealed it.
  • Step 6: We are also going to observe two other group's vials with the same combination as ours to see if there are any differences.
  • Step 7: At the end of three weeks we are going to count all the adult fruit flies and find out how many adult fruit flies there were at the end of three weeks. 
Data Table and Graph: The effect of the number of Fruit Flies on the number of adult fruit flies at the end of three weeks.






Analysis: I observed three vials for three weeks. Each vial had a different number of adults at the end of the three weeks. Vial 1 (ACOE) had 151 adults, Vial 2 (Elijah) had 105 adults and Vial 3 (18) had 70 adults.
When first starting this project I hypothesized that if I put four females and two males in a vial then I expect to see 646 adults at the end of three weeks because each females lays 20 eggs each day. The eggs take 16 days to become adult flies. After three weeks a female would have 160 eggs, but we have 4 females and six fruit flies. We multiply the eggs a female would have after three weeks (160) by the number of female fruit flies in the vial (4), then add the fruit flies we started with (6). This would give us 646 adult fruit flies at the end of three weeks.
My data did not support my hypothesis because the results weren't the same. I predicted to see 646 adults at the end of three weeks but there were only 151 adult fruit flies at the end of three weeks, thats lower than what I predicted.
Some possible reasons for the difference in my hypothesis and actual results could be carrying capacity. Carrying Capacity is the amount of living organisms an ecosystem can support. Another reason could have been a human made error. Someone could have done something to affect our results , like shake the vial and kill some flies or let some loose. Also maybe they could have been affected by the temperature of the place they were kept in. It might have been to cold or too hot.

Conclusion: Well overall we were trying to find out how many adult fruit flies there would be at the end of those three weeks of observation. After setting up my experiment I observed it for those three weeks. In the beginning nothing was happening, everything looked the same. It was like at the end, on the third week that I noticed there were way more flies. The vial was full of flies flying around and eegs on the wall and pupa and larva crawling. My data didn't support my hypothesis because my hypothesis was wrong. There were more flies than i predicted there would be. Something that could have gone wrong would be that some flies might have been killed or let loose by others. If i could do this over i would chose to put them in a bigger space to see what the difference would be. One question i have would have to be how does the temperature of the room where they are kept affect them ?